Tying a molecular knot with optical tweezers

YASUHARU ARAI, RYOHEI YASUDA, KEN-ICHIROU AKASHI, YOSHIE HARADA, HIDETAKE MIYATA, KAZUHIKO KINOSITA JR & HIROYASU ITOH

Nature 399 , 446 - 448 (1999).

Figure 1 Tying a knot in an actin filament. Explanatory drawings are added in images 3–7. In images 3 and 7, the microscope focus was moved to below the filament to bring down the end beads which were trapped at the focus level; the focus was above the filament in image 5. Scale bar, 10 µm.